FastQCFastQC Report
Fri 10 Feb 2017
ERR1147512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848843
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111580.6035125751618715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.3980868034765527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.2815815080025724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.10509275260257361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81600.051.786611
GGTATCA45500.050.4923021
AACGCAG101350.040.851836
TCAACGC107600.038.7000774
ATCAACG109800.037.979873
CAACGCA109650.037.868045
TATCAAC112850.037.2696762
ACGCAGA119850.034.6442957
CGCAGAG123050.033.6950048
GCAGAGT133200.031.306049
GTACATG103850.029.2862431
TACATGG106950.028.6460152
ACATGGG107500.028.1674213
AGAGTAC128750.026.88995210-11
TAGATCG1150.001006273625.864155
CATGGGG58300.025.8153514
AGTACTT77000.024.91456412-13
GAGTACT74400.024.46599212-13
CAGAGTA132450.024.1177410-11
ATGGGGG25200.023.6061695
Produced by FastQC (version 0.11.5)