FastQCFastQC Report
Fri 10 Feb 2017
ERR1147511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3403132
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184680.5426765697010871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115300.3388055473604903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.2710444378883922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67350.057.0103841
GTATCAA126950.052.0970541
AACGCAG160200.040.9561656
TCAACGC166700.039.2878274
ATCAACG169300.038.688453
CAACGCA171300.038.163365
TATCAAC183500.035.9539342
ACGCAGA187450.035.03357
CGCAGAG194250.033.8372278
GCAGAGT210250.031.3188029
GTACATG168350.029.4553181
TACATGG173450.028.2963622
ACATGGG174900.027.6195853
AGAGTAC198750.027.30984710-11
GAGTACT108200.025.4809712-13
CAGAGTA205650.025.29441310-11
AGTACTT113950.024.92612-13
CATGGGT40000.024.0913584
CATGGGG94700.023.6808684
GTACTTT129950.021.26194414-15
Produced by FastQC (version 0.11.5)