FastQCFastQC Report
Fri 10 Feb 2017
ERR1147510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470220
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249041.0081693128547256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143560.5811628114095101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125220.5069184121252358No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42530.1721708997579163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.13059565544769292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188700.047.1280561
AACGCAG215550.040.953196
GGTATCA111650.040.2521481
TCAACGC242100.036.6094634
ATCAACG244350.036.323253
CAACGCA245100.036.088565
ACGCAGA247900.035.5369727
TATCAAC252250.035.3271752
CGCAGAG251350.035.072868
GCAGAGT264850.033.3524939
GTACATG222150.031.3234121
ACATGGG224150.030.307883
TACATGG228550.030.2709982
CATGGGT40900.029.8148374
CATGGGG116650.029.1684364
ATGGGAG22500.027.4949345
AGAGTAC262550.027.09698910-11
AGTACTT138250.026.95614612-13
ATATGTA12800.026.5073151
GAGTACT131850.026.3923212-13
Produced by FastQC (version 0.11.5)