FastQCFastQC Report
Fri 10 Feb 2017
ERR1147509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898459
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179101.993413166321446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117361.3062365672779725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93691.042785480472676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36880.4104806118030984No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.2790333226112711No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20630.2296153747694664No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18150.20201255705602594No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.1872094330403502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.17351932586795835No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA14550.1619439506978059No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA14350.1597179170112381No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.15715797827168518No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13590.15125898900228057No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA13130.14613911152317466No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.1268839201343634No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10010.11141298601271732No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA9890.11007736580077665No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA9260.10306535968808817No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9010.10028281757987842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG856.1978426E-849.0016022
ATACGTA505.332581E-447.5989076
GTATCAA158400.038.385261
AACGCAG161400.037.2327276
GGTATCA106150.035.2316971
CATGGGT23750.032.8181954
CATGGGG80650.032.0178644
CGCAGAG188000.031.9611368
ACGCAGA188250.031.859037
ATGGGGG24650.031.6199675
TATCAAC195200.031.1836552
ATGGGTG16050.031.1394735
ATCAACG196150.030.9112833
TCAACGC198200.030.5898654
GTATCAC1951.4078978E-930.5393161
GCAGAGT198550.030.4126919
CAACGCA201750.029.9336385
ACATGGG157600.029.8264833
GTACATG159750.029.7105981
TACATGG161250.029.4834462
Produced by FastQC (version 0.11.5)