FastQCFastQC Report
Fri 10 Feb 2017
ERR1147508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665396
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227551.36634169891125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141980.8525299688482498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117300.7043369865185217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.2627603284744289No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.17701495620260885No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23270.1397265275045695No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.11726940619528328No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19320.11600844483834476No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.1064011202140512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186850.042.6265031
AACGCAG199350.039.745846
GGTATCA117650.039.0102841
TATCAAC228350.034.7013172
ACGCAGA231800.034.130447
TCAACGC233650.033.834744
CGCAGAG233700.033.8275038
ATCAACG233950.033.7943953
CAACGCA237900.033.1052745
GCAGAGT247250.032.190189
TATCACG1551.9746767E-730.7044322
GTACATG198750.030.60981
CATGGGG100200.029.8609714
TACATGG203400.029.7156732
ACATGGG199900.029.491963
CATGGGT33050.028.0773944
ATGGGGG38200.027.7178975
ATGGGAG21050.027.6934855
GTAAGTA13150.027.5960462
AGAGTAC245600.026.2061110-11
Produced by FastQC (version 0.11.5)