FastQCFastQC Report
Fri 10 Feb 2017
ERR1147507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023506
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182471.7827936524065322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123471.2063436853325726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98860.9658956566937567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.33355935382889795No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24760.24191357940256336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.18524561653766564No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18770.183389252236919No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.1649233126137023No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA16560.16179680431770796No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA15500.15144024558722666No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14090.13766406840800152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.13688244133400293No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12980.1268189927562711No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.10962319712830212No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.1086461632858039No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10810.10561735837405935No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA10640.10395640084181236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA166000.038.6606981
TACAACG1104.7058893E-737.8569832
AACGCAG172700.036.7891356
GGTATCA110000.035.8822631
ATGGGTG18250.032.597015
CGCAGAG198450.032.2253768
ACGCAGA198500.032.097387
CATGGGG82050.031.8292644
CATGGGT28250.031.587374
ATGGGGG22800.031.3102865
TATCAAC207550.030.898452
ACATGGG163000.030.8396723
GTACATG165250.030.8023361
GCAGAGT210850.030.4712879
TACATGG167150.030.465482
ATCAACG210200.030.3391043
TCAACGC212700.029.926574
CAACGCA215200.029.5512665
TGGGGTG41650.027.852256
ATGGGGT51550.027.6963945
Produced by FastQC (version 0.11.5)