FastQCFastQC Report
Fri 10 Feb 2017
ERR1147506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735312
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252800.6767841615372424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140850.37707693493876815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134290.3595148142912828No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41820.11195851912771945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.1083175916764115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168550.054.494951
GGTATCA91850.048.9961241
AACGCAG207200.044.3222586
TCAACGC221000.041.50084
ATCAACG222050.041.2275053
CAACGCA226700.040.4573255
TATCAAC235150.039.110972
ACGCAGA245100.037.420147
CGCAGAG251000.036.6116338
GCAGAGT271100.034.1165549
GTACATG220850.031.0239451
TACATGG225100.030.2332382
ACATGGG225150.029.7749563
CATGGGG121550.027.8922924
AGAGTAC259950.027.720310-11
AGTACTT149250.026.1628312-13
GAGTACT143550.026.12439312-13
CATGGGT58800.025.6933484
CAGAGTA267050.025.65808510-11
ATGGGGG51650.025.1044395
Produced by FastQC (version 0.11.5)