FastQCFastQC Report
Fri 10 Feb 2017
ERR1147505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881130
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328010.8451404616696684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181430.4674669490586505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171850.442783416170033No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64800.16696168383950036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.13521835135643484No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42230.10880851710713117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.057.2171441
GTATCAA199350.056.879531
AACGCAG265950.042.4013566
ATCAACG272050.041.4778253
TATCAAC272950.041.342662
TCAACGC274550.041.0731774
CAACGCA280600.040.1876035
ACGCAGA312700.036.1002127
CGCAGAG319550.035.382198
ATGGGGG89800.034.043035
GTACATG288550.033.6235661
GCAGAGT341550.033.2076269
TACATGG294700.032.7808382
ACATGGG293950.032.3573073
CATGGGG163550.031.8562744
TATAACG3950.028.613482
AGAGTAC333300.028.21226710-11
TGGGGGG81750.027.4280556
AGTACTT181150.026.46294212-13
CATGGGT56300.026.0933574
Produced by FastQC (version 0.11.5)