FastQCFastQC Report
Fri 10 Feb 2017
ERR1147504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735635
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246400.659593348921937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139430.37324310324750676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128950.345188970549853No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.12161252370748213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.10651468893508065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91850.054.315851
GTATCAA171750.053.4848631
AACGCAG219300.041.8215756
TCAACGC232050.039.3186344
ATCAACG232400.039.240653
CAACGCA237850.038.4598665
TATCAAC246800.037.241332
ACGCAGA260650.035.255387
CGCAGAG265350.034.6757478
TATCACG3350.033.740432
GTACATG253450.032.861481
GCAGAGT282150.032.77979
TACATGG260400.031.9380232
ACATGGG258450.031.6947693
ATGGGGG67700.029.9585195
CATGGGG141700.029.3820974
CATGGGT63150.028.1612834
AGAGTAC275050.027.89540510-11
AGTACTT144900.025.73675312-13
CAGAGTA281100.025.24260910-11
Produced by FastQC (version 0.11.5)