FastQCFastQC Report
Fri 10 Feb 2017
ERR1147503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277804
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256701.1269626359423375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152610.6699874089254387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141830.6226611244865669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.17626626347130833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.16507127039903344No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT28800.12643756881628093No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27810.12209127738822129No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT25180.11054506884701229No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.10997434370999437No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24640.10817436443170703No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23690.10400368073811442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA177250.052.2251
GGTATCA99050.051.5063321
AACGCAG221900.041.437686
ATCAACG239200.038.570133
TATCAAC244100.038.089142
TCAACGC243550.037.8762444
CAACGCA248600.037.1068345
ACGCAGA259950.035.418037
CGCAGAG262350.035.094028
GCAGAGT275500.033.4405259
GTACATG228900.032.6386531
TACATGG231350.032.2946172
ACATGGG230750.031.8880843
ATGGGGG45250.031.4139545
CATGGGG115700.031.2031614
CATGGGT58350.030.8848174
ATGGGTG29750.030.3878425
AGAGTAC274950.027.94808610-11
TATCACG1505.307533E-627.7598272
AGTACTT146400.027.219312-13
Produced by FastQC (version 0.11.5)