FastQCFastQC Report
Fri 10 Feb 2017
ERR1147502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401616
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255360.7507020192755444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152810.4492276612057328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140330.41253921665467236No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.11491596935103786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.11168221221913349No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT36460.1071843500265756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93750.055.7486841
GTATCAA175000.054.5944331
AACGCAG227400.041.8232846
ATCAACG240800.039.551123
TCAACGC241300.039.4880184
CAACGCA247050.038.544875
TATCAAC254450.037.5473982
ACGCAGA267700.035.6160137
CGCAGAG271300.035.033798
GCAGAGT287700.033.202129
GTACATG247400.032.385961
TACATGG251900.031.6456032
ACATGGG251800.031.161123
ATGGGGG63550.029.762665
CATGGGG139950.029.5798854
AGAGTAC282000.028.57921810-11
CATGGGT63250.028.023084
AGTACTT148800.026.0818112-13
GAGTACT144700.025.91652112-13
CAGAGTA286800.025.8196510-11
Produced by FastQC (version 0.11.5)