FastQCFastQC Report
Fri 10 Feb 2017
ERR1147501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828375
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247320.8744243602775444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146520.5180359747204667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133950.4735934944977239No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.13587307199363594No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33490.11840721262208866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171850.052.9239041
GGTATCA93650.051.227751
AACGCAG211250.042.79446
ATCAACG231100.039.2264373
TCAACGC231950.039.0778474
CAACGCA236650.038.3017435
TATCAAC238600.038.1686062
ACGCAGA246150.036.678547
CGCAGAG250550.036.081898
GCAGAGT266700.033.9638569
GTACATG221250.031.3146631
TACATGG223600.031.0456392
ACATGGG225550.030.4338443
CATGGGT55800.029.3115374
AGAGTAC259100.028.78526310-11
CATGGGG114850.028.0160144
ATGGGTG30700.027.1225035
AGTACTT142400.026.60538512-13
GAGTACT137500.026.32073212-13
CAGAGTA264200.026.21481510-11
Produced by FastQC (version 0.11.5)