FastQCFastQC Report
Fri 10 Feb 2017
ERR1147500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913614
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405290.824830766112275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226600.4611676863506169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217670.4429936905910802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.13307923658634968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62810.12782851888650593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA276650.052.8892061
GGTATCA151450.049.0131951
AACGCAG339950.042.5860826
TCAACGC371050.039.2731554
ATCAACG373550.039.0127033
CAACGCA380300.038.2715
TATCAAC384250.038.0981672
ACGCAGA398000.036.3298877
CGCAGAG406100.035.6199048
GCAGAGT437200.033.276569
GTACATG351000.032.0192151
TACATGG359750.031.003222
ACATGGG360700.030.4585653
CATGGGG184650.029.7957674
CATGGGT82800.029.4520594
ATGGGGG80050.029.1264045
AGAGTAC423850.028.17822610-11
AGTACTT239050.026.24979212-13
CAGAGTA431000.025.55104310-11
GAGTACT232950.025.37966212-13
Produced by FastQC (version 0.11.5)