FastQCFastQC Report
Fri 10 Feb 2017
ERR1147498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4968082
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353430.7114013013472805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196790.3961085988516293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187520.3774494865422914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65370.13157995379303322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.12079108195074074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA243200.053.286741
GGTATCA131400.051.7815631
AACGCAG304200.042.4680186
ATCAACG323600.039.798983
TCAACGC323900.039.7932434
CAACGCA332450.038.7877245
TATCAAC344750.037.6000672
ACGCAGA362300.035.591997
CGCAGAG369100.035.0168468
GTACATG337400.033.1871
GCAGAGT394800.032.7976469
TACATGG345400.032.4484672
ACATGGG344500.032.0487633
CATGGGT93700.030.7867264
CATGGGG180500.029.8217224
ATGGGGG80600.029.5179585
TATCACG3950.028.6148952
AGAGTAC382550.027.76863910-11
AGTACTT209950.025.5677612-13
CAGAGTA388850.025.42203110-11
Produced by FastQC (version 0.11.5)