FastQCFastQC Report
Fri 10 Feb 2017
ERR1147497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212341
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244200.7601932671531447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141660.44098680681783164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117110.36456279081205883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44740.1392753758084836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.11960125030312785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81500.060.188921
GTATCAA155600.057.1976971
AACGCAG208800.042.5282256
TCAACGC210700.042.0882644
ATCAACG211550.041.840683
CAACGCA215650.041.122185
TATCAAC218900.040.6550872
ACGCAGA239550.037.044237
CGCAGAG245100.036.181148
GCAGAGT261200.034.2014669
GTACATG229850.033.9292071
TACATGG235000.032.9082342
ACATGGG235100.032.1083343
CATGGGG133000.031.9296764
ATGGGGG72200.031.7978695
AGAGTAC254350.028.38800610-11
CATGGGT54600.027.3419234
GAGTACT127050.027.33922212-13
CAGAGTA257400.026.94250310-11
TGGGGGG64800.026.525946
Produced by FastQC (version 0.11.5)