FastQCFastQC Report
Fri 10 Feb 2017
ERR1147495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316153
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257900.7777083867963872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149740.4515473200422297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128650.3879495306760575No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.12194853494395463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40110.12095340594960487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA174750.056.1808851
GGTATCA90500.054.273831
AACGCAG223350.043.7812586
TCAACGC234450.041.7084434
ATCAACG234200.041.707833
CAACGCA239150.040.8390055
TATCAAC247000.039.765612
ACGCAGA259900.037.71587
CGCAGAG264750.037.0922748
GCAGAGT281650.034.9722029
GTACATG241950.032.727371
TACATGG247600.031.8363132
ACATGGG248250.031.3915923
CATGGGT65700.030.9622944
CATGGGG127650.029.2158874
AGAGTAC276300.028.74983810-11
GAGTACT139650.027.0035412-13
AGTACTT145900.026.72328212-13
CAGAGTA280850.026.39916610-11
TATAACG3101.2732926E-1124.9481982
Produced by FastQC (version 0.11.5)