FastQCFastQC Report
Fri 10 Feb 2017
ERR1147494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6806324
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441980.6493666772254744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263300.3868461154655582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228770.3361138846754871No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83850.12319425287423873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70790.10400621539615217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156500.056.260061
GTATCAA301750.055.398271
AACGCAG393150.042.1776056
TCAACGC402200.041.125044
ATCAACG407750.040.526873
CAACGCA412450.040.117445
TATCAAC423550.039.241512
ACGCAGA456400.036.3715327
CGCAGAG466800.035.573948
GCAGAGT498950.033.3890049
GTACATG427250.030.8072571
TACATGG440900.029.7233922
ACATGGG438950.029.2036443
AGAGTAC484100.027.91390810-11
CATGGGG246650.027.7791374
ATGGGGG129550.027.1329755
CATGGGT85950.026.2265224
CAGAGTA496050.025.6947310-11
GAGTACT249800.025.27405712-13
AGTACTT262250.025.07209612-13
Produced by FastQC (version 0.11.5)