FastQCFastQC Report
Fri 10 Feb 2017
ERR1147493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389041
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297860.6786448338031018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182530.4158767256901907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154470.35194476424348736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52680.1200262198507601No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45670.10405462149932068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107550.055.412891
GTATCAA198800.053.487491
AACGCAG263150.040.231686
ATCAACG268450.039.3785483
TCAACGC269200.039.305424
CAACGCA275500.038.3202445
TATCAAC284350.037.387962
ACGCAGA306800.034.4883277
CGCAGAG313800.033.737948
GCAGAGT336000.031.632749
GTACATG274800.029.876841
TACATGG281750.029.1601772
ACATGGG279400.028.8501823
CATGGGT76500.028.4558284
AGAGTAC321300.027.23036610-11
TATAACG4650.025.5880662
CATGGGG141350.025.162684
CAGAGTA329700.025.04831310-11
AGTACTT177700.025.03604312-13
GAGTACT171000.025.025712-13
Produced by FastQC (version 0.11.5)