FastQCFastQC Report
Fri 10 Feb 2017
ERR1147492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707275
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243120.8980247666011026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146610.5415408482699393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130490.4819975805930317No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.13740754079286369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.12998310108873315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.053.9124151
GTATCAA155800.053.2621571
AACGCAG197300.041.627546
ATCAACG209850.039.257913
TCAACGC210850.039.0088164
TATCAAC212550.038.983822
CAACGCA215250.038.1561625
ACGCAGA228050.036.066697
CGCAGAG231000.035.657598
GCAGAGT250100.033.0057759
GTACATG200250.031.778151
TACATGG207050.030.7399332
ACATGGG207550.030.2927473
CATGGGG113400.029.3165554
AGAGTAC241200.028.37998810-11
ATGGGGG52550.028.1799895
CATGGGT47450.026.69674
AGTACTT135950.026.26926812-13
CAGAGTA246950.025.91298310-11
GAGTACT130650.025.30926512-13
Produced by FastQC (version 0.11.5)