FastQCFastQC Report
Fri 10 Feb 2017
ERR1147491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406930
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288950.8481242643670401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180660.5302721218222857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149600.43910500068977054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.12289656670374796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.10149900350168628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA210700.056.366721
GGTATCA107850.054.1493451
AACGCAG269200.044.1437726
ATCAACG283400.041.8923953
TCAACGC283250.041.8701254
TATCAAC289150.041.1863822
CAACGCA290850.040.8373955
ACGCAGA312850.038.0797277
CGCAGAG316700.037.5980268
GCAGAGT336450.035.4440089
GTACATG282450.031.4675251
CATGGGT81150.030.856214
TACATGG287900.030.8276142
ACATGGG288050.030.3545633
CATGGGG139350.029.2369864
AGAGTAC331000.028.7771610-11
AGTACTT176400.027.17599712-13
ATGGGGG60750.026.8258385
ATGGGTG47450.026.8241885
CAGAGTA334300.026.58050710-11
Produced by FastQC (version 0.11.5)