FastQCFastQC Report
Fri 10 Feb 2017
ERR1147489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900823
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283070.975826515440618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167470.5773189194928474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146310.5043741034871828No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47750.16460845766873747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47210.16274691699562505No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC44880.1547147137208992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA204500.053.7371671
GGTATCA109500.051.647091
AACGCAG260800.041.9863556
TCAACGC276850.039.5737344
ATCAACG277800.039.423793
TATCAAC281000.039.1892432
CAACGCA283950.038.6051645
ACGCAGA300750.036.4091157
CGCAGAG304250.036.0293738
ATGGGGG88950.035.3061145
GCAGAGT318850.034.510189
GTACATG282350.034.3876531
TACATGG288150.033.5919842
ACATGGG285500.033.5472223
CATGGGG167250.032.8600164
CATGGGT52500.032.5150574
TGGGGGG80650.030.2371886
AGAGTAC320850.028.37221310-11
AGTACTT159450.027.04428112-13
TAACGCA4000.026.7654464
Produced by FastQC (version 0.11.5)