FastQCFastQC Report
Fri 10 Feb 2017
ERR1147488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3508785
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252650.7200498178144287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147950.42165592933166324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128850.36722113210128293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.10693160167978374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA184500.052.519671
GGTATCA102100.048.3856161
AACGCAG229350.042.0334746
TCAACGC246300.039.164944
ATCAACG247500.039.0057533
CAACGCA251050.038.376545
TATCAAC261900.037.1574132
ACGCAGA268600.035.9354867
CGCAGAG273400.035.2610748
GCAGAGT291900.033.168929
GTACATG246950.032.3691181
TACATGG253300.031.4443842
ACATGGG253950.030.7774353
CATGGGT68400.029.8228424
AGAGTAC282150.028.24462110-11
CATGGGG122050.028.0669824
GAGTACT145050.025.7485612-13
CAGAGTA284850.025.60721610-11
AGTACTT151250.025.32220512-13
ATGGGTA28650.023.8717485
Produced by FastQC (version 0.11.5)