FastQCFastQC Report
Fri 10 Feb 2017
ERR1147487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065536
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205910.9968841017537337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120180.5818344487823015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101370.4907684978620561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.14465978806469604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.13521913924521287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144250.055.7833861
GGTATCA75650.055.386991
AACGCAG186900.043.1148576
ATCAACG194050.041.346363
TATCAAC195900.041.016622
TCAACGC197400.040.670884
CAACGCA200750.040.0218125
ACGCAGA215150.037.4813547
CGCAGAG218650.036.881388
GCAGAGT231550.034.955089
GTACATG201700.032.6357231
TACATGG205850.031.9264322
ACATGGG204400.031.5127683
CATGGGT57650.030.9470214
CATGGGG97550.030.786554
ATGGGGG45450.030.0947595
ATGGGTG30250.029.2925915
AGAGTAC228150.028.92036210-11
AGTACTT114550.027.80105612-13
GAGTACT110350.026.97296512-13
Produced by FastQC (version 0.11.5)