FastQCFastQC Report
Fri 10 Feb 2017
ERR1147486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231676
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226450.7007199979205837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139610.43200494108939136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107900.33388248079324784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.10920030349577123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105300.050.259531
GTATCAA182800.050.119581
AACGCAG220150.041.36366
TCAACGC239050.038.1430324
ATCAACG239500.038.100333
CAACGCA243800.037.375495
TATCAAC248850.036.839542
ACGCAGA258150.035.2748267
CGCAGAG263100.034.6337668
GCAGAGT280200.032.5201459
GTACATG242600.031.7041951
TACATGG249400.030.69952
ACATGGG248050.030.4560343
CATGGGG140150.029.5802294
ATGGGGG65450.028.535265
CATGGGT47450.027.5770824
AGAGTAC272450.027.17968210-11
GAGTACT134400.026.59726712-13
CAGAGTA276150.026.07243310-11
AGTACTT139350.025.18296612-13
Produced by FastQC (version 0.11.5)