FastQCFastQC Report
Fri 10 Feb 2017
ERR1147485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655676
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343130.9386225693961938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201110.5501308102796856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170170.4654953010058878No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.13494084267861814No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.11825446237576853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA230050.054.4664341
GGTATCA123700.052.932461
AACGCAG292050.042.710766
ATCAACG306700.040.5564653
TCAACGC308500.040.3176234
TATCAAC314300.039.8412972
CAACGCA316650.039.355065
TATCACG2450.038.8489842
ACGCAGA337600.036.9304737
CGCAGAG341200.036.488528
GCAGAGT363750.034.3573049
GTACATG305550.031.8713251
TACATGG313050.031.1451912
CATGGGT100800.030.7449114
ACATGGG313500.030.6256853
CATGGGG146400.029.4979344
AGAGTAC358300.028.34718510-11
ATGGGTG54200.028.2052465
ATGGGGG65950.026.968235
AGTACTT192850.026.69583512-13
Produced by FastQC (version 0.11.5)