FastQCFastQC Report
Fri 10 Feb 2017
ERR1147484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834377
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211930.7477128130802642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123150.434487014253926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113220.39945286036402355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35360.1247540464800554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.11035934880927979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143150.052.436781
GGTATCA78850.050.12781
AACGCAG180600.041.135056
TCAACGC190800.038.9671864
ATCAACG192750.038.605873
CAACGCA196000.037.9940575
TATCAAC198200.037.7263642
ACGCAGA209900.035.4213377
CGCAGAG213550.034.788068
GCAGAGT229350.032.4952359
GTACATG184900.030.1980021
TACATGG189350.029.4365982
ACATGGG186550.029.334793
AGAGTAC222000.027.9982410-11
CATGGGT38700.027.2038084
CATGGGG105850.026.8599034
ATGGGGG47650.025.714125
AGTACTT129150.025.28397412-13
CAGAGTA227700.025.11618210-11
TAACGCA3600.024.7831294
Produced by FastQC (version 0.11.5)