FastQCFastQC Report
Fri 10 Feb 2017
ERR1147482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432905
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193530.7954687914242439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116060.47704287672556056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98870.40638660366927604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.1253645333459383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.12281613955333233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136950.057.1281621
GGTATCA70300.051.74911
AACGCAG171950.045.2782866
ATCAACG180100.043.072173
TCAACGC180900.042.8737564
CAACGCA184450.042.0808375
TATCAAC190300.041.049082
ACGCAGA202100.038.464657
CGCAGAG207200.037.5465938
GCAGAGT218850.035.5750549
GTACATG190950.032.3975941
TACATGG194550.031.8955842
ACATGGG193250.031.552753
CATGGGG102250.030.7130644
ATGGGGG49000.030.5884155
CATGGGT49650.029.7087884
AGAGTAC213250.028.60218210-11
AGTACTT111000.027.0059912-13
GAGTACT106750.026.68825312-13
GTAAGTA17350.026.403942
Produced by FastQC (version 0.11.5)