FastQCFastQC Report
Fri 10 Feb 2017
ERR1147481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301819
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178680.776255648250362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111650.4850511704004528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.4027249753347244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.1265955316208616No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.1118680487040901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71450.053.1466981
GTATCAA132350.052.3014341
AACGCAG171600.040.3780756
ATCAACG178400.038.709853
TCAACGC179850.038.459734
CAACGCA184250.037.6058465
TATCAAC187050.037.2075582
ACGCAGA200000.034.822827
CGCAGAG201250.034.488318
GTACATG194800.033.640021
TACATGG198550.032.895422
ACATGGG195650.032.5911063
GCAGAGT214900.032.2976889
ATGGGGG46650.032.0006685
CATGGGG99100.031.628164
CATGGGT51350.031.0406884
TATGTAA13650.027.8901162
AGAGTAC210200.027.19115410-11
ATATGTA13750.026.8377951
GAGTACT99600.026.54296312-13
Produced by FastQC (version 0.11.5)