FastQCFastQC Report
Fri 10 Feb 2017
ERR1147480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534854
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169900.6702555650147898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97350.3840457872524414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87340.34455633342196434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.11172241083707386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.11148571081411394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120000.055.1625981
GGTATCA63000.051.0239871
AACGCAG152750.043.0669066
ATCAACG158500.041.545353
TCAACGC160250.041.162654
CAACGCA164550.040.123145
TATCAAC165850.039.8850862
ACGCAGA178700.036.8129277
CGCAGAG184550.035.6460088
GCAGAGT197300.033.402779
GTACATG168250.031.7364641
TACATGG172000.031.1960262
ACATGGG172200.030.3986023
ATGGGGG39250.028.7927915
AGAGTAC187800.028.07714710-11
CATGGGT35150.027.9208354
CATGGGG92000.027.4124494
AGTACTT100450.025.52096712-13
GAGTACT95900.025.4913612-13
CAGAGTA194650.025.24035510-11
Produced by FastQC (version 0.11.5)