FastQCFastQC Report
Fri 10 Feb 2017
ERR1147479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226293
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177630.5505699575333052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104950.32529593561403136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.2772221865775985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137350.056.209471
GGTATCA71600.050.5462271
AACGCAG174750.043.7327776
TCAACGC181050.042.3424034
ATCAACG183550.041.7410473
CAACGCA185600.041.272335
TATCAAC194900.039.5562632
ACGCAGA201950.037.8719987
CGCAGAG208850.036.6777348
GCAGAGT224000.034.2501759
GTACATG200700.032.8914031
TACATGG207050.031.8336622
ACATGGG207700.031.2743283
CATGGGT51050.030.5229644
AGAGTAC216450.027.99869210-11
ATGGGGG49850.027.6785895
CATGGGG109200.027.3946914
GAGTACT106300.025.70830212-13
CAGAGTA221350.025.56527110-11
AGTACTT112600.024.87732312-13
Produced by FastQC (version 0.11.5)