FastQCFastQC Report
Fri 10 Feb 2017
ERR1147478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329198
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219800.6602190677754822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132220.3971527076491095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117770.3537488608367541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.11435186492362426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152550.053.8154341
GGTATCA81600.051.7262341
AACGCAG187900.043.5527656
TCAACGC200350.040.786974
ATCAACG203150.040.2326623
CAACGCA204150.040.0277755
TATCAAC214350.038.297552
ACGCAGA219900.037.269037
CGCAGAG224850.036.4221158
GCAGAGT243300.033.63579
GTACATG206200.032.4801981
TACATGG213850.031.4885442
ACATGGG210200.031.3273583
CATGGGG110500.030.0866364
CATGGGT48450.029.215094
AGAGTAC233650.028.635910-11
ATGGGGG49750.027.1366885
AGTACTT130050.026.31837512-13
CAGAGTA239350.025.97853710-11
GAGTACT125400.025.34978312-13
Produced by FastQC (version 0.11.5)