FastQCFastQC Report
Fri 10 Feb 2017
ERR1147477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6712729
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389480.5802111183097068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205440.3060454250424827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198730.2960494904531376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA255850.056.1176571
GGTATCA132000.054.0695651
AACGCAG325900.043.5990456
TCAACGC332350.042.8065954
ATCAACG337900.042.0729943
CAACGCA342050.041.627445
TATCAAC360750.039.573562
ACGCAGA379400.037.4353757
CGCAGAG390250.036.364098
GCAGAGT422750.033.737339
GTACATG364900.033.0175061
TACATGG378550.031.8515572
ACATGGG378550.031.3168283
CATGGGG208500.029.8951264
AGAGTAC405750.028.2394110-11
ATGGGGG96800.027.8335155
CAGAGTA418000.025.54784810-11
GAGTACT215550.025.219912-13
AGTACTT225800.025.02331712-13
CGTATAC4050.023.4995023
Produced by FastQC (version 0.11.5)