FastQCFastQC Report
Fri 10 Feb 2017
ERR1147476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125525
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208850.6682077410994953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.3667543852632758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109370.3499252125642892No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.11988385951160205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.10673406867646236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134150.058.7052461
GGTATCA65750.056.2216451
AACGCAG173700.045.3379946
TCAACGC177550.044.2878844
ATCAACG178800.043.9492153
CAACGCA182250.043.080485
TATCAAC192150.041.144742
ACGCAGA203250.038.717157
CGCAGAG207500.037.895498
GCAGAGT225250.035.0413069
GTACATG194400.033.896831
TACATGG201200.032.6119882
ACATGGG197850.032.5016563
CATGGGG105300.030.107384
AGAGTAC217350.028.9534410-11
CATGGGT55350.028.1551484
ATGGGGG52350.027.837075
AGTACTT120050.026.55686412-13
GAGTACT115300.025.89694612-13
CAGAGTA224050.025.85759410-11
Produced by FastQC (version 0.11.5)