FastQCFastQC Report
Fri 10 Feb 2017
ERR1147475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4263632
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289990.6801478176352931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168360.394874604562495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148880.34918585844181677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.12407262165215009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.10481673840519068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93950.060.5797231
GTATCAA182700.057.350911
AACGCAG246850.042.3131526
TCAACGC247850.042.0944334
ATCAACG249050.041.898493
CAACGCA254250.041.0348325
TATCAAC263050.039.8066062
ACGCAGA286950.036.4000787
CGCAGAG292400.035.701288
GCAGAGT314350.033.302999
GTACATG274200.033.0238651
TACATGG282600.031.8949012
ACATGGG280500.031.7076193
ATGGGGG83200.031.0992535
CATGGGG152350.031.039074
AGAGTAC304450.028.12416810-11
CATGGGT72000.028.0886214
TGGGGGG72700.027.0818026
GAGTACT154700.026.4149912-13
CAGAGTA309500.026.1758310-11
Produced by FastQC (version 0.11.5)