FastQCFastQC Report
Fri 10 Feb 2017
ERR1147473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826931
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181250.6411546656073318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108610.38419756265717137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92170.32604262360842906No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.14305266028778205No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39840.14093021725680607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.11510716037993146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.068.29671
GTATCAA119550.060.20111
ATCAACG165250.043.0184143
TCAACGC165600.042.9214254
AACGCAG168200.042.222596
CAACGCA170100.041.7165
TATCAAC175450.040.7562752
ACGCAGA196000.036.3552637
CGCAGAG199950.035.607328
GTACATG206350.035.5990521
TACATGG210350.034.870962
ATGGGGG79150.034.5679365
ACATGGG211300.034.431423
GCAGAGT213450.033.466749
CATGGGG125350.033.3578074
TGGGGGG69150.031.7395466
CATGGGT49100.030.2848224
AGAGTAC206700.028.28652410-11
TATCACG1505.3049553E-627.7619632
AGTACTT95650.026.80148312-13
Produced by FastQC (version 0.11.5)