FastQCFastQC Report
Fri 10 Feb 2017
ERR1147472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055182
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167400.5479215313523057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95600.3129109820626071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83840.2744190035159935No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.10388906454672749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119100.060.7231141
GGTATCA55900.059.0977171
TCAACGC159900.044.8974044
ATCAACG160150.044.8710673
AACGCAG160950.044.60456
CAACGCA164300.043.7312435
TATCAAC174300.041.3989942
ACGCAGA188550.038.1383747
CGCAGAG191800.037.5541538
GCAGAGT205100.035.20599
GTACATG188350.033.2144131
TACATGG191150.032.334612
ACATGGG192150.031.8567433
ATGGGGG49600.030.2190825
CATGGGG101200.030.0332814
TATCACG2103.1486707E-928.3274252
AGAGTAC197900.028.16152810-11
CATGGGT49750.028.095534
AGTACTT100700.026.3726912-13
CAGAGTA201150.026.22804810-11
Produced by FastQC (version 0.11.5)