FastQCFastQC Report
Fri 10 Feb 2017
ERR1147470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921351
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123830.644494420852827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69580.3621410143175297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62930.3275299515809449No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.13454074763018314No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22000.1145027639405814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.11023493364825063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.061.015451
GTATCAA83350.058.993341
AACGCAG113100.043.2335856
TCAACGC114150.042.679564
ATCAACG115600.042.2000733
CAACGCA116250.042.062095
TATCAAC122050.040.2157332
ACGCAGA132800.036.8201687
TATCACG1304.208414E-836.612252
CGCAGAG136400.035.9792068
GGGTTAG6100.035.131871
GTACATG137150.034.9838681
GCAGAGT144600.034.1857229
TACATGG140350.033.869922
ATGGGGG48550.033.326655
ACATGGG142350.032.9743923
CATGGGG78550.031.8821744
TCTAACG1203.6994512E-529.7474582
TGGGGGG43400.029.4686836
AGAGTAC141750.028.3684510-11
Produced by FastQC (version 0.11.5)