FastQCFastQC Report
Fri 10 Feb 2017
ERR1147469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032135
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247630.6141411435877023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135900.33704228653058493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123930.30735578049842077No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.10984255239469909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.10269993440199794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162500.059.194771
GGTATCA80500.057.8237461
ATCAACG219450.043.3135763
TCAACGC219200.043.2788854
AACGCAG220550.042.9330676
CAACGCA224800.042.17435
TATCAAC236250.040.6635322
ACGCAGA257800.036.7757347
CGCAGAG265600.035.695728
GCAGAGT284500.033.387099
GTACATG248150.032.214921
TACATGG256600.031.1331982
ACATGGG255750.030.9095153
CATGGGG137100.028.8496234
ATGGGGG71300.027.8620345
AGAGTAC275300.027.48127210-11
CATGGGT68950.026.3963014
AGTACTT142300.025.89359312-13
CAGAGTA281450.025.19015910-11
GAGTACT138800.024.7681912-13
Produced by FastQC (version 0.11.5)