FastQCFastQC Report
Fri 10 Feb 2017
ERR1147468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369949
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198630.8381193013014204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.4772254592820352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99280.41891196814783777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40850.17236657835252994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32800.13839960269187226No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29010.12240769738082971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62350.061.1007461
GTATCAA127850.060.0609251
AACGCAG167850.045.216116
ATCAACG172800.044.0278363
TCAACGC173050.043.9605184
CAACGCA176700.042.951475
TATCAAC180700.042.201742
ACGCAGA195650.038.760937
CGCAGAG199250.038.0307548
GCAGAGT212750.035.89719
GTACATG190600.035.0094871
ATGGGGG58000.034.8670165
TACATGG193150.034.523232
GGGTTAG8400.034.014571
ACATGGG195850.033.4398463
CATGGGG107350.032.2466664
CATGGGT51450.030.7510624
AGAGTAC207750.029.80393210-11
TGGGGGG51050.027.8462056
CAGAGTA210750.027.13598410-11
Produced by FastQC (version 0.11.5)