FastQCFastQC Report
Fri 10 Feb 2017
ERR1147467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799003
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244310.8728465099894498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139860.49967792103116715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122610.43804883381689835No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.1619862501040549No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40900.14612345896020834No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29640.10589484898730012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153550.059.4050141
GGTATCA76300.057.6678921
AACGCAG201050.044.9120336
ATCAACG206350.043.9689143
TCAACGC207250.043.7406734
CAACGCA212200.042.6642725
TATCAAC213250.042.6585962
ACGCAGA235500.038.34217
CGCAGAG237750.037.954228
GCAGAGT252950.035.7910969
GTACATG224350.033.8109631
TACATGG228450.032.970892
CATGGGT58300.032.241384
ACATGGG231900.032.0693133
ATGGGGG70250.030.7365675
CATGGGG128300.029.9502814
GCGTATC2001.8517312E-929.7703651
AGAGTAC248200.028.78301410-11
AGTACTT126100.027.45382912-13
GAGTACT122050.026.8783612-13
Produced by FastQC (version 0.11.5)