FastQCFastQC Report
Fri 10 Feb 2017
ERR1147464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003659
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169190.8444051607583926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101650.507321854666887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86740.4329079948234705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.13834689435677427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.1346536511452298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.056.866691
GTATCAA119000.055.181331
AACGCAG154050.042.55156
ATCAACG158750.041.2229423
TCAACGC161300.040.5282944
TATCAAC163000.040.2576072
CAACGCA164750.039.7157025
ACGCAGA179350.036.5158127
CGCAGAG182950.035.797278
GCAGAGT193200.033.9904569
GTATTAC3050.033.1827771
GTACATG168950.030.973791
CATGGGT37000.030.5454734
TACATGG172450.030.3584442
ACATGGG172450.029.9444663
ATGGGGG39050.028.4849575
CATGGGG85550.028.4379524
ATGGGTG21450.028.008455
AGAGTAC190400.027.7578310-11
CAGAGTA193950.026.11499210-11
Produced by FastQC (version 0.11.5)