FastQCFastQC Report
Fri 10 Feb 2017
ERR1147463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039815
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181360.8891002370312994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103770.5087226047460186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91550.44881521118336715No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.19643938298326072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32330.1584947654566713No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24010.11770675281827028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122600.053.516191
GGTATCA66100.051.3413661
AACGCAG149150.043.1480676
ATCAACG160900.040.4456563
TATCAAC161600.040.3460542
TCAACGC161200.040.2916374
CAACGCA164700.039.4715275
ACGCAGA176100.036.5785457
CGCAGAG180600.035.7659238
GCAGAGT193500.033.658179
ATGGGGG44150.033.544865
CATGGGG93950.031.780764
GTACATG165150.031.544491
TACATGG169300.030.6753232
ACATGGG166900.030.5802863
GGGTTAG5850.028.4968071
TGGGGGG40500.027.6096046
AGAGTAC189700.027.01128810-11
CAGAGTA192900.025.71527510-11
GAGTACT100250.025.3931612-13
Produced by FastQC (version 0.11.5)