FastQCFastQC Report
Fri 10 Feb 2017
ERR1147462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407073
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173850.7222464794378899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101610.4221309449277193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.36430137349386577No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41880.1739872450897833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40550.1684618621869798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30470.1265852759762583No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24480.10170028079746647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.055.6289521
GTATCAA122650.055.5745771
AACGCAG161650.042.169186
ATCAACG166400.040.8275343
TCAACGC167800.040.4818534
TATCAAC170700.040.113562
TATCACG758.198838E-539.66052
CAACGCA172000.039.631675
ATGGGGG75200.037.1763085
GTACATG200100.036.8606071
ACGCAGA187850.036.224397
TACATGG204250.035.9711462
ACATGGG204800.035.4671333
CGCAGAG191500.035.4097068
CATGGGG120250.035.3182684
GGGTTAG12500.034.765711
GCAGAGT204450.033.3704879
TGGGGGG66900.033.253076
GTTAGAG14000.031.8693793
CATGGGT41900.030.3798754
Produced by FastQC (version 0.11.5)