FastQCFastQC Report
Fri 10 Feb 2017
ERR1147461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084929
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152000.7290416124481937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.4286956534251286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.37267456110016217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.1162149886159193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.1019698992147934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114100.049.923831
GGTATCA65000.047.8011971
AACGCAG137600.041.2813576
ATCAACG145800.038.8817753
TCAACGC146900.038.66794
CAACGCA151100.037.553725
TATCAAC154250.036.9454882
ACGCAGA161300.035.215847
CGCAGAG163100.034.827198
GCAGAGT174600.032.5673689
GTACATG143350.030.6851561
TACATGG145750.030.080152
ACATGGG146650.029.975963
CATGGGT28650.029.2727494
CATGGGG77450.027.8775274
AGAGTAC170350.027.02513310-11
ATGGGGG31350.025.4235575
AGTACTT97250.025.35151512-13
CAGAGTA172950.025.3463810-11
GAGTACT94200.024.49908412-13
Produced by FastQC (version 0.11.5)