FastQCFastQC Report
Fri 10 Feb 2017
ERR1147460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305043
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168460.7308323532359267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113310.4915743437324162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81010.35144680598149364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.10659237159567089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63850.064.7923661
GTATCAA118950.053.945381
AACGCAG159200.040.4227646
TCAACGC161200.039.6998674
ATCAACG161050.039.6647033
TATCAAC162700.039.4452362
CAACGCA165350.038.847355
ACGCAGA185150.034.7251247
CGCAGAG187850.034.2576758
GCAGAGT200650.032.0722859
GTACATG162750.030.1008511
TACATGG169750.028.9421582
ACATGGG168500.028.7332783
CATGGGG90450.028.2091854
GAGTACT100450.027.20679712-13
AGAGTAC194650.026.94982710-11
CAGAGTA198900.026.59824610-11
ATGGGGG49150.026.0169735
AGTACTT104600.025.4166112-13
GTAAGTA23250.025.3263662
Produced by FastQC (version 0.11.5)