FastQCFastQC Report
Fri 10 Feb 2017
ERR1147459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241474
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141880.6329763361074008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92390.4121841252675695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72120.32175256103795985No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.1543627095384555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.1109091606683816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.058.8326151
GTATCAA102400.056.9012371
AACGCAG131200.044.1553236
TCAACGC135200.042.7609674
ATCAACG136500.042.400133
CAACGCA137950.041.9516535
TATCAAC144300.040.4398082
ACGCAGA152400.038.0910387
CGCAGAG157800.036.7498478
GGGTTAG7650.035.787831
GCAGAGT168250.034.6087239
GTACATG144400.032.148921
TACATGG146650.031.5170332
ACATGGG151000.030.0168463
CATGGGG82400.028.800744
ATGGGGG42550.028.0966435
AGAGTAC163300.028.08187710-11
CAGAGTA165650.026.80379910-11
GAGTACT82700.026.6465312-13
CATGGGT35800.026.5824414
Produced by FastQC (version 0.11.5)