FastQCFastQC Report
Fri 10 Feb 2017
ERR1147458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083446
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295570.7238249262999927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171270.41942516198328567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140550.3441945846718678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.11429072406002186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201350.056.7135771
GGTATCA104600.054.073191
AACGCAG256050.044.2770356
TCAACGC268900.042.271764
ATCAACG272050.041.7660643
CAACGCA273800.041.47185
TATCAAC282350.040.3502732
ACGCAGA301400.037.6149147
CGCAGAG308900.036.6823778
GCAGAGT330050.034.4038169
GTACATG279450.031.5104161
TACATGG285350.030.8775422
ACATGGG285200.030.474713
CATGGGT77000.028.5046734
AGAGTAC319250.028.38520210-11
CATGGGG145850.028.3030664
AGTACTT171150.026.7083712-13
CAGAGTA325300.026.1384910-11
GAGTACT164500.025.9620512-13
ATGGGGG61150.025.2904935
Produced by FastQC (version 0.11.5)