FastQCFastQC Report
Fri 10 Feb 2017
ERR1147456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855077
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164540.8869712685780697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98070.5286573010176936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77390.4171794486158796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.13719107077495976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.12802703068390153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.056.2171
GTATCAA115600.055.163111
AACGCAG145650.043.7372066
ATCAACG150400.042.5220953
TCAACGC150300.042.4632074
CAACGCA154150.041.3640755
TATCAAC156050.040.9455032
ACGCAGA167800.037.8928957
CGCAGAG170250.037.382538
GCAGAGT181650.035.1019679
TATCACG1102.042089E-532.4507142
GTACATG161250.032.4199141
TACATGG163100.031.9897882
CATGGGT57400.031.8125514
ACATGGG163350.031.1751563
ATGGGTG29450.030.6994325
TAGCGTG1004.448361E-429.7400745
AGAGTAC177450.027.63673410-11
CATGGGG73150.027.5649624
GAGTACT86750.027.32315812-13
Produced by FastQC (version 0.11.5)