FastQCFastQC Report
Fri 10 Feb 2017
ERR1147455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141805
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148800.6947411178888834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.41329626179787604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74210.34648345671057823No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.15225475708572908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.1124752253356398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.066.505751
GTATCAA94450.062.2202721
TCAACGC127700.045.741594
ATCAACG128600.045.4257133
AACGCAG129650.045.0077366
CAACGCA130250.044.754745
TATCAAC136400.043.1789782
ACGCAGA151950.038.4416057
CGCAGAG155900.037.5057688
GCAGAGT164550.035.7510729
GTACATG148100.033.167721
TACATGG152800.032.0036852
ACATGGG153300.031.5873723
CATGGGG80150.029.7599054
CATGGGT44250.029.4388964
AGAGTAC160400.028.94404410-11
ATGGGGG41350.028.9142025
ATGGGTG24550.027.1367115
CAGAGTA164200.026.87951710-11
GAGTACT76750.026.73815212-13
Produced by FastQC (version 0.11.5)