FastQCFastQC Report
Fri 10 Feb 2017
ERR1147452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478782
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344370.9899154359198133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216490.6223155115784778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169820.4881593615236597No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.17106562009346948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.1552554888463836No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT35700.10262212464017578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.057.996751
GTATCAA225300.055.0906371
AACGCAG289350.042.5710956
ATCAACG298350.041.312183
TCAACGC299500.041.1482284
CAACGCA306700.040.162855
TATCAAC309550.040.048622
ACGCAGA335100.036.7590147
CGCAGAG338900.036.417058
GCAGAGT357250.034.61319
GTACATG305250.031.61261
TACATGG311000.031.0058232
ACATGGG310400.030.4903813
CATGGGG154250.030.1922044
CATGGGT75850.028.3080434
AGAGTAC353400.028.25806210-11
ATGGGGG70900.027.8515935
GAGTACT177050.027.36234912-13
CAGAGTA357600.026.89494910-11
AGTACTT182450.025.94940612-13
Produced by FastQC (version 0.11.5)